Mac Mania 2

home *** CD-ROM | disk | FTP | other *** search

/ Mac Mania 2 / MacMania 2.toast / Demo's / Tools&Utilities / Scientific / CC Converter / CC test doc < prev next >

Wrap

Text File | 1993-10-28 | 17.2 KB | 301 lines | [TEXT/ttxt]

/H:Help /P:Previous step /A:Database menu /S:Search menu DOCUMENTS 1 TO 7 PAGE = 1 OF 13 CAUL/ISI09 DOCUMENT= 1 OF 7 PUBDATE = 9309 INDATE = 930922 RT I UI LU957-0021 TI KINETIC PCR ANALYSIS - REAL TIME MONITORING OF DNA AMPLIFICATION REACTIONS AU HIGUCHI R (Reprint). FOCKLER C. DOLLINGER G. WATSON R. AD ROCHE MOLEC SYST INC, 1145 ATLANTIC AVE, ALAMEDA, CA, 94501 USA (Reprint). CHIRON CORP, EMERYVILLE, CA, 94608 USA SO BIO-TECHNOLOGY, 1993 SEP, v11, n9 p.1026-1030 AB We describe a simple, quantitative assay for any amplifiable DNA sequence that uses a video camera to monitor multiple polymerase chain reactions (PCRs) simultaneously over the course of thermocycling. The video camera detects the accumulation of double-stranded DNA (dsDNA) in each PCR using the increase in the fluorescence of ethidium bromide (EtBr) that results from its binding duplex DNA. The kinetics of fluorescence accumulation during thermocycling are directly related to the starting number of DNA copies. The fewer cycles necessary to produce a detectable fluorescence, the greater the number of target sequences. Results ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 2 OF 13 obtained with this approach indicate that a kinetic approach to PCR analysis can quantitate DNA sensitively, selectively and over a large dynamic range. This approach also provides a means of determining the effect of different reaction conditions on the efficacy of the amplification and so can provide insight into fundamental PCR processes. KP POLYMERASE CHAIN REACTION. ENZYMATIC AMPLIFICATION. SEQUENCES. INVITRO JS BIOTECHNOLOGY & APPLIED MICROBIOLOGY RE 18 REFS LA ENGLISH DO ARTICLE IS 0733-222X TC For table of contents see UI LU957-0000 AA Y GA LU957 CAUL/ISI09 DOCUMENT= 2 OF 7 PUBDATE = 9309 INDATE = 930922 RT I UI LU957-0024 ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 3 OF 13 TI A REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION ASSAY FOR THE DETECTION AND QUANTITATION OF MURINE RETROVIRUSES AU IRVING J M. CHANG L W S. CASTILLO F J (Reprint). AD XOMA CORP, 2910 7TH ST, BERKELEY, CA, 94710 USA (Reprint). XOMA CORP, 2910 7TH ST, BERKELEY, CA, 94710 USA SO BIO-TECHNOLOGY, 1993 SEP, v11, n9 p.1042-1046 AB Specific hybridization primers for the PCR assay were developed to detect the presence of the ecotropic, xenotropic, and mink cell focus-forming classes of murine leukemia viruses (MuLVs) in samples derived from cultured cells and cell-free supernatants. The primers, which were tested against reference viruses from all three classes and two subclasses and accurately identified each class present, were used to characterize the endogenous expression of MuLV-related sequences in a number of murine and mink cell lines. Two murine/murine hybridomas were shown to contain expressed retroviral sequences from all three classes. The murine cell lines SC-1, Balb/c 3T3, and NIH 3T3, were found to constitutively express sequences from many of the MuLV classes. These MuLV-related sequences were not expressed in the Mus dunni or mink lung cell lines. When these primers were used in a quantitative PCR assay to determine the ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 4 OF 13 retroviral content of hybridoma supernatants, the values were less variable than those obtained by transmission electron microscopy (TEM). This assay can be adapted to detect and quantitate any viral contaminant in cell culture supernatants, ascites fluids, process validation samples, and final products. KP LEUKEMIA VIRUSES. SEQUENCES. PROBES. MICE. RNA JS BIOTECHNOLOGY & APPLIED MICROBIOLOGY RE 34 REFS LA ENGLISH DO ARTICLE IS 0733-222X TC For table of contents see UI LU957-0000 AA Y GA LU957 CAUL/ISI08 DOCUMENT= 3 OF 7 PUBDATE = 9300 INDATE = 930814 RT I UI LB968-0037 TI APPLICATION OF THE POLYMERASE CHAIN REACTION TECHNIQUE TO THE DETECTION OF PATHOGENS IN WATER ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 5 OF 13 AU TORANZOS G A (Reprint). ALVAREZ A J. DVORSKY E A. AD UNIV PUERTO RICO, DEPT BIOL, RIO PIEDRAS, PR, 00931 USA (Reprint) SO WATER SCIENCE AND TECHNOLOGY, 1993 v27, n3-4 p.207-210 AB Enteric pathogens may be present in fecally contaminated waters at extremely low concentrations. In addition, these pathogens may be injured when exposed to the environment and may not be able to grow in laboratory culture media or such media may simply not exist for their progagation in the laboratory. It is paramount thus to use techniques which do not depend on culture techniques for the detection of these pathogens and that allow for the detection of single-cell concentrations. The polymerase chain reaction (PCR) technique has been shown to be an excellent and sensitive means of detecting pathogens in waters. Membrane filtration has been combined with PCR and DNA hybridization techniques to be able to detect the DNA equivalent of one single cell in large volumes of water. In addition, this combination of methods allows for the amplification of different target genes that may be present in the sample, since the membrane can be subjected to repeated amplification reactions under different conditions. A Most Probable Number PCR was developed which allows for the quantification of gene copy number and thus permits ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 6 OF 13 extrapolation to estimate the number of bacterial cells in the original sample. AK MPN PCR. ENTERIC PATHOGENS. WATER MICROBIOLOGY. QUANTITATIVE PCR KP DNA JS WATER RESOURCES. ENVIRONMENTAL SCIENCES. ENGINEERING, CIVIL RE 14 REFS LA ENGLISH DO ARTICLE IS 0273-1223 TC For table of contents see UI LB968-0000 AA Y GA LB968 CAUL/ISI08 DOCUMENT= 4 OF 7 PUBDATE = 9307 INDATE = 930814 RT I UI LM932-0002 TI AN ELECTROCHEMILUMINESCENCE BASED DETECTION SYSTEM FOR QUANTITATIVE PCR AU ANDERSON M S (Reprint). DICESARE J L. KATZ E D. AD PERKIN ELMER CORP, INSTRUMENT MARKETING, 761 MAIN AVE, NORWALK, CT, ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 7 OF 13 06859 USA (Reprint) SO AMERICAN BIOTECHNOLOGY LABORATORY, 1993 JUL, v11, n8 p.10-10 JS BIOTECHNOLOGY & APPLIED MICROBIOLOGY RE NO REFS LA ENGLISH DO ARTICLE IS 0749-3223 TC For table of contents see UI LM932-0000 AA N GA LM932 CAUL/ISI08 DOCUMENT= 5 OF 7 PUBDATE = 9302 INDATE = 930812 RT I UI KP837-0008 TI USING RANDOMLY AMPLIFIED POLYMORPHIC DNA FOR EVALUATING GENETIC RELATIONSHIPS AMONG PAPAYA CULTIVARS AU STILES J I (Reprint). LEMME C. SONDUR S. MORSHIDI M B. MANSHARDT R. AD UNIV HAWAII, COLL TROP AGR & HUMAN RESOURCES, DEPT PLANT MOLEC PHYSIOL, HONOLULU, HI, 96822 USA (Reprint). UNIV HAWAII, COLL TROP ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 8 OF 13 AGR & HUMAN RESOURCES, DEPT HORT, HONOLULU, HI, 96822 USA SO THEORETICAL AND APPLIED GENETICS, 1993 FEB, v85, n6-7 p.697-701 AB We have applied the recently developed technique of random amplification of polymorphic DNA (RAPD) to the analysis of the relationships among ten cultivars of papaya (Carica papaya L.). Eleven ten-base synthetic oligonucleotides were chosen that gave multiple PCR amplification products using papaya DNA as template. These 11 primers amplified a total of 102 distinct fragments. Cultivars were scored for presence or absence of RAPD fragments and grouped by cluster analysis using simple matching coefficients of similarity. A dendrogram of the ten cultivars was constructed. Of the ten cultivars seven were of the Hawaiian type, and all of these grouped to one branch of the tree. Divisions within the Hawaiian, branch were mostly consistent with the known genetic background of these cultivars. Three non-Hawaiian, cultivars were also analyzed. The minimum similarity detected was 0.7 suggesting that the domesticated papaya germ plasm is quite narrow. Our results show that RAPD technology is a rapid, precise and sensitive technique for genomic analysis. AK CARICA PAPAYA L. POLYMERASE CHAIN REACTION. GENETIC POLYMORPHISM. ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 9 OF 13 PHYLOGENETIC RELATIONSHIP KP QUANTITATIVE TRAITS. MENDELIAN FACTORS. PCR. PRIMERS JS GENETICS & HEREDITY RE 16 REFS LA ENGLISH DO ARTICLE IS 0040-5752 TC For table of contents see UI KP837-0000 AA Y GA KP837 CAUL/ISI08 DOCUMENT= 6 OF 7 PUBDATE = 9303 INDATE = 930812 RT I UI KT810-0036 TI HIGH LEVELS OF HIV 1 IN PLASMA DURING ALL STAGES OF INFECTION DETERMINED BY COMPETITIVE PCR AU PIATAK M. SAAG M S. YANG L C. CLARK S J. KAPPES J C. LUK K C. HAHN B H. SHAW G M. LIFSON J D (Reprint). AD GENELABS TECHNOL INC, DIV HIV & EXPLORATORY RES, REDWOOD CITY, CA, 94063 USA (Reprint). GENELABS TECHNOL INC, DIV HIV & EXPLORATORY ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 10 OF 13 RES, REDWOOD CITY, CA, 94063 USA. UNIV ALABAMA, DEPT MED, DIV HEMATOL ONCOL, BIRMINGHAM, AL, 35294 USA. UNIV ALABAMA, DEPT MED, DIV INFECT DIS, BIRMINGHAM, AL, 35294 USA SO SCIENCE, 1993 MAR 19, v259, n5102 p.1749-1754 AB Quantitative competitive polymerase chain reaction (QC-PCR) methods were used to quantify virion-associated human immunodeficiency virus type-1 (HIV-1) RNA in plasma from 66 patients with Centers for Disease Control stage I to IVC1 infection. HIV-1 RNA, ranging from 100 to nearly 22,000,000 copies per milliliter of plasma (corresponding to 50 to 11,000,000 virions per milliliter), was readily quantified in all subjects, was significantly associated with disease stage and CD4+ T cell counts, and decreased by as much as 235-fold with resolution of primary infection or institution of antiretroviral therapy. Plasma virus levels determined by QC-PCR correlated with, but exceeded by an average of 60,000-fold, virus titers measured by endpoint dilution culture. Quantitation of HIV-1 in plasma by QC-PCR may be useful in assessing the efficacy of antiretroviral agents, especially in early stage disease when conventional viral markers are often negative. KP HUMAN IMMUNODEFICIENCY VIRUS. POLYMERASE CHAIN REACTION. T CELL ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 11 OF 13 RECEPTOR. HTLV III. LEUKEMIA VIRUS. SAN FRANCISCO. MESSENGER RNA. AIDS. DISEASE. QUANTITATION JS MULTIDISCIPLINARY SCIENCES RE 71 REFS LA ENGLISH DO ARTICLE IS 0036-8075 TC For table of contents see UI KT810-0000 AA Y GA KT810 CAUL/ISI08 DOCUMENT= 7 OF 7 PUBDATE = 9300 INDATE = 930812 RT I UI KW281-0002 TI REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION - AN OVERVIEW OF THE TECHNIQUE AND ITS APPLICATIONS AU OHAN N W (Reprint). HEIKKILA J J. AD UNIV WATERLOO, DEPT BIOL, WATERLOO N2L 3G1, ONTARIO, CANADA (Reprint) SO BIOTECHNOLOGY ADVANCES, 1993 v11, n1 p.13-29 AB The polymerase chain reaction (PCR) has galvanized molecular ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 12 OF 13 biologists by virtue of its ability to provide them with large quantities of any desired fragment (up to 11kb) of DNA. This power combined with its flexibility has also inspired many useful applications, including new methods of DNA sequencing, cloning and mutagenesis. One logical variation of PCR has been its application to the detection and analysis of messenger RNA by the addition of a reverse transcription step prior to performing PCR. Due to the exquisite sensitivity of PCR, reverse transcription-PCR (RT-PCR) has been used to characterize mRNAs previously undetectable by established methods of RNA analysis such as Northern hybridization and RNase protection assays. Furthermore, its capacity as a method of quantitative analysis is currently being developed. RT-PCR has also been used to diagnose the presence of certain diseases. Recently, RT-PCR has been employed to identify and isolate genes that are differentially expressed in different cells or environmental conditions. AK REVERSE TRANSCRIPTION. POLYMERASE CHAIN REACTION (PCR). RT PCR. MESSENGER RNA. CDNA. PRIMER DESIGN. CONTAMINATION. QUANTITATION KP MESSENGER RNA. DNA AMPLIFICATION. RIBONUCLEIC ACID. GENERAL METHOD. PCR. INVITRO. HYBRIDIZATION. CONTAMINATION. ------------------------- Press ENTER to see more ------------------------- /H:Help /P:Previous step /A:Database menu /S:Search menu /U:Scroll up /F:Print offline /Q:Quit /O:Other options /N:STAIRS Cmd: __ DOCUMENTS 1 TO 7 PAGE = 13 OF 13 QUANTITATION. TEMPERATURE JS BIOTECHNOLOGY & APPLIED MICROBIOLOGY RE 53 REFS LA ENGLISH DO REVIEW. NONARTICLE IS 0734-9750 TC For table of contents see UI KW281-0000 AA Y GA KW281 R0601 * END OF D